KAJIAN HISTOPATOLOGI ORGAN TRAKHEA DAN PARU-PARU AYAM BROILER YANG DITANTANG VIRUS AVIAN INFLUENZA SETELAH PEMBERIAN EKSTRAK ADAS BINTANG (Illicium verum)
(Study on Histopathological Trachea and Lungs of Broiler that Challenged by Avian influenza Virus after Treatment with Star Anise Extract (Illicium verum))
ZUHRA TAUFIKA, AGUS SETIYONO, WIWIN WINARSIH
ABSTRAK
Penelitian ini bertujuan untuk mengetahui gambaran histopatologi organ trakhea dan paru-paru ayam yang ditantang virus Avian Influenza (AI) H5N1 setelah pemberian ekstrak adas bintang (Illicium verum). Penelitian ini menggunakan 14 ekor ayam broiler strain Cobb dibagi menjadi 4 kelompok perlakuan, yaitu kelompok kontrol positif (KP) yang tidak diberi adas bintang namun diuji tantang AI, kontrol negatif (KN) yang tidak diberi adas bintang dan tidak ditantang AI, perlakuan 1 yang diberi adas bintang dan tidak diuji tantang AI (P1), dan perlakuan 2 yang diberi adas bintang dan diuji tantang AI (P2). Hasil penelitian ini menunjukkan perbedaan yang nyata antara lesio histopatologi trakhea kelompok KP, P1 dan KN. Sebaliknya, lesio histopatologi paru-paru kelompok KP dan P2 juga berbeda nyata dengan paru-paru KN. Dapat disimpulkan bahwa bahan aktif pada ekstrak adas bintang 1 ml/hari per ekor selama 3 minggu dapat mengurangi kerusakan organ trakhea dan paru-paru akibat ditantang virus Avian Influenza H5N1 dengan dosis 104,0 EID50/0,1 ml per ekor.
Kata kunci : Avian Influenza, ayam broiler, ekstrak adas bintang, paru-paru, trachea
ABSTRACT
The objective of this research was to study the histopathology of trachea and lungs of broiler that challenged by avian influenza virus after treatment with stars anise extract (illicium verum). This experiment used 14 heads of Cobb broiler, and were divided into 4 group treatments, they included: positive control (KP) who were not given star anise but challenged by AI virus, negative control (KN) who were not given star anise and not challenged by AI virus, first treatment which were given the star anise and were not challenged by AI virus (P1), and the second treatment which were not given the star anise and were challenged by AI virus AI (P2). The results showed histopathologically significant difference between the trachea of KP, P1 and KN. In contrast, lung of KP and P2 were also histopathology significantly different from KN. It concluded that the active ingredient of the star anise extract at 1 ml/day per head during 3 weeks can reduced the damage of trachea and lung of broiler after challenging with avian influenza H5N1 104,0 EID50/0.1 ml per head.
Keywords: Avian Influenza, broiler, star anise extract, lung, trachea