Departemen
Klinik, Reproduksi dan Patologi
Bagian
Reproduksi dan Kebidanan
Email
Karja_nwk@yahoo.com
Latar Belakang Pendidikan
-Â Fakultas Kedokteran Hewan, UGM, Yogyakarta, Indonesia (S1)
– Fakultas Kedokteran Hewan, UGM, Yogyakarta, Indonesia (S2)
– United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi, Japan (S3)
Kepakaran
Bioteknologi Reproduksi (Produksi embrio in vitro dan cloning)
Riset Interest
-Â Bioteknologi Reproduksi (Produksi embrio in vitro dan cloning)
– Preservasi gamet dan embrio
Publikasi
Ni Wayan Kurniani Karja, Medvedev S, Onishi A, Fuchimoto D, Iwamoto M, Otoi T, Nagai T: Effect of replacement of pyruvate/lactate in culture medium with glucose on preimplantation development of porcine embryos in vitro. Journal of Reproduction and Development (2004), 50:587-592.
Ni Wayan Kurniani Karja, Wongsrikeao P, Murakami M, Agung B, Fahrudin M, Nagai T, Otoi T: Effects of oxygen tension on the development and quality of porcine in vitro fertilized embryos. Theriogenology (2004), 62:1585-1595.
Ni Wayan Kurniani Karja, Otoi T, Murakami M, Wongsrikeao P, Budiyanto A, Fahrudin M, Nagai T: Effect of cycloheximide on in vitro development of electrically activated feline oocytes. Journal of Reproduction and Development (2005), 51:783-786.
Ni Wayan Kurniani Karja, Otoi T, Wongsrikeao P, Murakami M, Agung B, Fahrudin M, Nagai T: In vitro development and post-thaw survival of blastocysts derived from delipidated zygotes from domestic cats. Theriogenology (2006), 65:415-423.
Ni Wayan Kurniani Karja, Otoi T, Wongsrikeao P, Shimizu R, Murakami M, Agung B, Fahrudin M, Nagai T: Effects of electric field strengths on fusion and in vitro development of domestic cat embryos derived by somatic cell nuclear transfer. Theriogenology (2006), 66: 1237-42.
Ni Wayan Kurniani Karja, Kikuchi K, Fahrudin M, Ozawa M, Somfai T, Ohnuma K, Noguchi J, Kaneko H, Nagai T: Development to the blastocyst stage, the oxidative state, and the quality of early developmental stage of porcine embryos cultured in alteration of glucose concentrations in vitro under different oxygen tensions. Reproductive Biology and Endocrinology (2006), 4:54.
Murakami M, Otoi T, Ni Wayan Kurniani Karja, Wongsrikeao P, Agung B, Suzuki T: Blastocysts derived from in vitro-fertilized cat oocytes after vitrification and dilution with sucrose. Cryobiology (2004), 48:341-348.
Murakami M, Ni Wayan Kurniani Karja, Wongsrikeao P, Agung B, Taniguchi M, Naoi H, Otoi T: Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol. Reproduction in Domestic Animals (2005), 40:511-515.
Agung B, Otoi T, Abe H, Hoshi H, Murakami M, Ni Wayan Kurniani Karja, Murakami MK, Wongsrikeao P, Watari H, Suzuki T: Relationship between oxygen consumption and sex of bovine in vitro fertilized embryos. Reproduction in Domestic Animals (2005), 40:51-56.
Wongsrikeao P, Otoi T, Ni Wayan Kurniani Karja, Agung B, Nii M, Nagai T: Effects of ovary storage time and temperature on DNA fragmentation and development of porcine oocytes. Journal of Reproduction and Development (2005), 51:87-97.
Wongsrikeao P, Otoi T, Murakami M, Ni Wayan Kurniani Karja, Budiyanto A, Nii M, Suzuki T: Relationship between DNA fragmentation and nuclear status of in vitro-matured porcine oocytes: role of cumulus cells. Reproduction Fertility and Development (2005), 16:773-780.
Rajaei F, Ni Wayan Kurniani Karja, Agung B, Wongsrikeao P, Taniguchi M, Murakami M, Sambuu R, Nii M, Otoi T: Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants. Reproduction in Domestic Animals (2005), 40:429-432.
Wongsrikeao P, Otoi T, Taniguchi M, Ni Wayan Kurniani Karja, Agung B, Nii M, Nagai T: Effects of hexoses on in vitro oocyte maturation and embryo development in pigs. Theriogenology (2006), 65:332-343.
Noguchi J, Ohnuma K, Ozawa M, Ni Wayan Kurniani Karja, Fahrudin M, Somfai T, Kikuchi K, Kaneko H: Successful Long Term Culture of Immature Porcine Sertoli Cells in the Reconstructed Testicular Cell Cord. Journal of Reproduction and Development (2006), 51: 51-57.
Ozawa M, Nagai T, Fahrudin M, Ni Wayan Kurniani Karja, Kaneko H, Noguchi J, Ohnuma K, Kikuchi K: Addition of glutathione or thioredoxin to culture medium reduces intracellular redox status of porcine IVM/IVF embryos, resulting in improved development to the blastocyst stage. Molecular Reproductive and Development (2006), 73:998-1007.
Somfai T, Ozawa M, Noguchi J, Kaneko H, Ohnuma K, Ni Wayan Kurniani Karja, Fahrudin M, Maedomari N, Dinnyes A, Nagai T, Kikuchi K: Diploid porcine parthenotes produced by inhibition of first polar body extrusion during in vitro maturation of follicular oocytes. Reproduction (2006), 132:559-570.
Naoi H, Otoi T, Shimamura T, Ni Wayan Kurniani Karja, Agung B, Shimizu R, Taniguchi M, Nagai T. Developmental Competence of Cat Oocytes from Ovaries Stored at Various Temperature for 24 h. Journal of Reproduction and Development. (2006). J Reprod Dev. 53:271-7.
Fahrudin M, Kikuchi K, Ni Wayan Kuriani Karja, Ozawa M, Somfai T, Ohnuma K, Noguchi J, Kaneko H, and Nagai T: Development to the Blastocyst Stage of Porcine Somatic Cell Nuclear Transfer Embryos Reconstructed by the Fusion Of Cumulus Cells and Cytoplasts Prepared by Gradient Centrifugation, Cloning and Stem Cells. (2007). 9:216-228.
Somfai T, Ozawa M, Noguchi J, Kaneko H, Ohnuma K, Ni Wayan Kuriani Karja, Farhudin M, Maedomari N, Dinnyés A, Nagai T, Kikuchi K: Developmental competence of in vitro fertilized porcine oocytes after in vitro maturation and Solid Surface Vitrification: the effect of cryopreservation on oocyte antioxidative system Cryobiology. (2006). 55:115-126.
T. Somfai, K. Kikuchi, J. Noguchi, H. Kaneko, K. Ohnuma, M. Ozawa, Ni Wayan Kurniani Karja, M. Fahrudin, N. Maedomari, A. Dinnyes, and T. Nagai. 2006. Inhibition of first polar body extrusion by cytochalasin B during in vitro maturation of porcine oocytes leads to re-arrangement of the segregated chromosomes and improvement in the quality of parthenogenetic blastocysts. Reprod.Fertil.Dev. 18: 226.
Somfai, T., M. Ozawa, J. Noguchi, H. Kaneko, Ni Wayan Kurniani Karja, M. Fahrudin, M. Nakai, N. Maedomari, A. Dinnyes, T. Nagai, and K. Kikuchi. In vitro development of polyspermic porcine oocytes: Relationship between early fragmentation and excessive number of penetrating spermatozoa. Anim Reprod Sci. (2007). Accepted.
Ozawa, M., T. Nagai, T. Somfai, M. Nakai, N. Maedomari, M. Fahrudin, N.W. Karja, H. Kaneko, J. Noguchi, K. Ohnuma, N. Yoshimi, H. Miyazaki, and K. Kikuchi. 2008. Comparison between effects of 3-isobutyl-1-methylxanthine and FSH on gap junctional communication, LH-receptor expression, and meiotic maturation of cumulus-oocyte complexes in pigs. Mol Reprod Dev. 75:857-66.
Naoi, H., B. Agung, N.W. Karja, P. Wongsrikeao, R. Shimizu, M. Taniguchi, and T. Otoi. 2008. Effects of the reproductive status on morphological oocyte quality and developmental competence of oocytes after in vitro fertilization and somatic cell nuclear transfer in cat. Reprod Domest Anim. 43:157-61.
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