GAMBARAN HISTOPATOLOGI ORGAN HATI DAN GINJAL AYAM BROILER SETELAH PEMBERIAN EKTRAK SIRIH MERAH DAN DIUJI TANTANG VIRUS AI H5N1
(Histopathology of Liver and Kidney of Chicken Post Piper crocatum Extract Treatment and Challenged by Avian Influenza Virus)
ARIFIN BUDIMAN NUGRAHA. AGUS SETIYONO, WIWIN WINARSIH
ABSTRAK
Penelitian ini bertujuan untuk mengetahui gambaran histopatologi organ hati dan ginjal ayam broiler setelah pemberian ektrak ethanol sirih merah dan diuji tantang virus AI H5N1. Dalam penelitian ini terdapat empat perlakuan diantaranya adalah K1 (Kontrol Positif), K2 (Kontrol Negatif), P1 (Kelompok perlakuan yang diberi ekstrak sirih merah dan diuji tantang virus AI H5N1), dan P2 (kelompok perlakuan yang hanya diberi ekstrak sirih merah). Data hasil pengamatan histopatologi diolah dengan uji statistik Kruskall Wallis dan dilanjutkan dengan uji Dunn. Berdasarkan hasil data kematian, ekstrak sirih merah mampu menghambat kematian ayam sebesar 62.5%. lesio histopatologi yang terlihat adalah kongesti, degenerasi, dan nekrosis. Dapat disimpulkan bahwa ekstrak sirih merah mampu menghambat kematian akibat infeksi virus AI, namun masih menyisakan perubahan patologis pada organ hati dan ginjal.
Kata kunci : Ekstrak ethanol sirih merah, virus AI H5N1, hati, ginjal, ayam broiler.
ABSTRACT
The objective of this study was to determine the potency of Piper crocatum ethanolic extract (PC) to prevent virus infection, and to describe the liver and kidney histopathology of broiler after challenged by H5N1 virus. There were four group treatments in this study, such as K1 (Positive Control), K2 (Negative Control), P1 (Chickens PC treatment and challenged by H5N1 virus), and P2 (Chicken PC treatment). The histopathological data was processed by Kruskall Wallis statistical analysis followed by Dunn test. Based on mortality data, PC in a single composition capable to reduce mortality up to 62.5%. Histopathological lesion that appeared in liver and kidney were congestion, cell degeneration, and necrose. It can be concluded that PC able to prevent the death of chicken due to infection of H5N1 virus, however remain lesion in the liver and kidney.
Key words : Piper crocatum ethanolic extract, H5N1Virus, Liver, Kidney, Broiler Chicken