POTENSI EKSTRAK SAMBILOTO (Andrographis paniculata Nees) UNTUK MENANGGULANGI VIRUS AVIAN INFLUENZA H5N1 PADA AYAM BROILER: GAMBARAN HISTOPATOLOGI ORGAN PARU-PARU
(The Potency of Sambiloto (Andrographis paniculata Nees) Extract to Eliminate Avian Influenza H5N1 Virus Infection in Chicken : Histopathology of Lungs)
ARDHINTA IRAWAN, WIWIN WINARSIH, AGUS SETIYONO
ABSTRAK
Tujuan dalam penelitian ini adalah mengetahui potensi sambiloto (Andrographis paniculata Nees) untuk menanggulangi virus AI H5N1 melalui gambaran histopatologi organ paru-paru ayam. Ekstrak sambiloto diberikan pada ayam setiap hari sejumlah 1 ml per oral selama tiga minggu di Laboratorium Patologi. Ayam yang telah dipelihara selama empat minggu diuji tantang virus AI di fasilitas BSL-3 dengan dosis 104.0 x EID 50 / 0,1 ml intranasal. Hasil penelitian ini berupa hemorhagi dan vaskulitis pada paru-paru ayam setelah diuji tantang virus AI H5N1. Bahwa ekstrak sambiloto memiliki potensi dalam mengurangi kerusakan jaringan pada paru-paru ayam.
Kata kunci : ekstrak sambiloto, virus H5N1, paru-paru, histopatologi
ABSTRACT
The purpose of this research was to know the potency of sambiloto extract to eliminate the Avian Influenza H5N1 virus infection in chicken and to observe the histopathology of lungs. Sambiloto extract was given orally in Pathology Laboratory during three weeks and then chicken were infected with Avian Influenza H5N1 virus intranasal 104.0 x EID50 / 0.1 ml dosage in BSL 3 facility. The results showed that mild tissue destruction mostly found such as hemorrhage and vasculitis. This study concluded that sambiloto exctract has a potency to reduce the tissue destruction in lungs after challanging with Avian Influenza H5N1 virus.
Keywords : sambiloto extract, H5N1 virus, lungs, histopathology