PEMERIKSAAN PROFIL HORMON PROGESTERON SELAMA SIKLUS ESTRUS TIKUS (Rattus norvegicus) BETINA MENGGUNAKAN PERANGKAT INFRAMERAH.
(The Examination of Progesterone Hormone Profile on Female Rats (Rattus norvegicus) throughout Estrous Cycle by Infrared Spectroscopy)
Luthfiralda Sjahfirdi, Putri Krida Gita P., Pudji Astuti, Hera Maheshwari
ABSTRAK
Penelitian ini bertujuan memperoleh profil fluktuasi kadar hormon ovari sepanjang siklus estrus. Sampel darah yang berasal dari sepuluh ekor tikus betina diambil selama tiga siklus estrus dan dianalisis menggunakan perangkat inframerah. Kadar progesteron selama tiga siklus memiliki kisaran 6-11 ng/ml pada masa estrus dan meningkat menjadi 32-49 ng/ml pada saat non-estrus.
Kata kunci: kawin inframerah, progesteron, siklus estrus, tikus betina
ABSTRACT
The aim of the study was to obtain hormonal fluctuated profile of ovarian hormones throughout estrous cycle. Blood samples from ten female rats were collected during three consecutive estrous cycles and analyzed by infrared spectroscopy. The absorbance values of functional groups of progesterone were converted to ng/ml ranging between 12.135-39.387 ng/ml for ketone; 7.995-35.702 ng/ml for metil; and 7.542-39.249 ng/ml for metil ketone. The progesterone level during three cycles ranging between 6-11 ng/ml on estrous cycle and 32-49 ng/ml on non estrous cycle.
Key words: infrared spectroscopy, progesterone, estrous cycle, female rat
Publish in Jurnal Kedokteran Hewan Vol. 7 No. 1, Maret 2013 p 32-36